Biological Safety
Cabinet Certification
Fundamentals Course

Biological Safety
Cabinet Certification
Fundamentals Course

Welcome to
EAB Training!

We’re thrilled to embark on this journey together! This page serves as your comprehensive guide to all the essential information you need before attending EAB Training.

As always, if you have any other questions, please reach out to our
Training Center Manager, Derek Glenn, at dglenn@eabcoinc.com.

What's Included in the Course?

– 4 days of instruction and hands-on activities

– Resource Materials

– Use of NSF required instruments

– Completion certificate and NSF continuing education credits

What's Included in the Course?

– 4 days of instruction and hands-on activities

– Resource Materials

– Use of NSF required instruments

– Completion certificate and NSF continuing education credits

What Should I Bring
to EAB Training?

• Computer or tablet with internet capabilities to access the EAB Learning Management System (LMS).

• Calculator

What Should I Bring
to EAB Training?

• Computer or tablet with internet capabilities to access the EAB Learning Management System (LMS).

• Calculator

What About Meals
and Snacks?

Breakfast, lunch, and snacks will be included for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

What About Meals
and Snacks?

Breakfast, lunch, and snacks will be included for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

What Should I Bring
to EAB Training?

What Should I Bring
to EAB Training?

A calculator, safety glasses, and safety gloves is all you will need for our classes. Once you are at EAB Training, everything else is provided…

A calculator, safety glasses, and safety gloves is all you will need for our classes. Once you are at EAB Training, everything else is provided…

What About Meals
and Snacks?

Breakfast, lunch, and snacks will be included for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

What About Meals
and Snacks?

Breakfast, lunch, and snacks will be included for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

Biological Safety Cabinet Certification Fundamentals Course

Biological Safety Cabinet Certification Fundamentals

The Biological Safety Cabinet (BSC) Certification Fundamentals course is designed to equip professionals with the essential knowledge and hands-on experience required to perform field certification tests on biosafety cabinets. This course establishes the foundation for all professionals in the industry, ensuring a standardized approach to best practices. Additionally, it offers owners valuable insights into the proper certification process, helping make informed decisions about quality and compliance. This course provides participants with eight (8) completed test reports, including tests from B2 cabinets, ensuring they meet partial prerequisites for advanced certification.

Throughout the course, participants will gain expertise in:

  • Fundamentals of BSC components and operation
  • Discuss the purpose and function of various types of BSCs and HEPA filters
  • Proper BSC Install, Setup, Cleaning and Disinfecting
  • Documenting certification reports in compliance with NSF/ANSI 49 standards
  • Performing measurements and calculations
  • Understanding airflow dynamics and critical performance criteria for BSC certification
  • Applying safety and regulatory best practices for laboratory environments
  • BSC Design and Construction
  • BSC Lifespan and Decommissioning process
  • Instructional and practical training on the following NSF Primary Tests:
    • o Downflow Velocity Test
    • o Inflow Velocity Test – DIM Method
    • o Inflow Velocity Test – Constricted Access Opening Method
    • o HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • o Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • o Site Installation Assessment Test
    • o Airflow Pattern Test
  • Overview discussion on the following NSF Secondary Tests:
    • o Vibration Test
    • o Lighting Intensity Test
    • o Noise Level Test

Calculating Inflow/Downflow Velocity From Volume – TypeB2 Cabinet is introduced in the BSC Fundamentals course; however, the actual practice is offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

BSC Decontamination is referenced but not explored, in depth, in the BSC Fundamentals course. Due to its complexities, this is considered a more advanced skill. The BSC Decontamination instruction and practical are offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

If you have questions, please contact the EAB Training Center Manager, Derek Glenn at dglenn@eabcoinc.com.

Who Should Attend:

  • Field certification professionals
  • Laboratory safety and compliance personnel
  • HVAC and TAB professionals working in controlled environments
  • Industrial hygiene professionals
  • Architects and Design Engineers
  • Professionals that utilize BSCs
  • Equipment Manufacturers
  • Facility Engineers and Managers
  • Those pursuing NSF/ANSI 49 certification
  • BioSafety and EHS Professional
Prerequisites: None
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 15 Hours
eLearning: Online LMS Resources for six months
CE Credits: 4 credits
Throughout the course, participants will gain expertise in:
  • Fundamentals of BSC components and operation
  • Discuss the purpose and function of various types of BSCs and HEPA filters
  • Proper BSC Install, Setup, Cleaning and Disinfecting
  • Documenting certification reports in compliance with NSF/ANSI 49 standards
  • Performing measurements and calculations
  • Understanding airflow dynamics and critical performance criteria for BSC certification
  • Applying safety and regulatory best practices for laboratory environments
  • BSC Design and Construction
  • BSC Lifespan and Decommissioning process
  • Instructional and practical training on the following NSF Primary Tests:
    • Downflow Velocity Test
    • Inflow Velocity Test – DIM Method
    • Inflow Velocity Test – Constricted Access Opening Method
    • HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • Site Installation Assessment Test
    • Airflow Pattern Test
  • Overview discussion on the following NSF Secondary Tests:
    • Vibration Test
    • Lighting Intensity Test
    • Noise Level Test

Calculating Inflow/Downflow Velocity From Volume – TypeB2 Cabinet is introduced in the BSC Fundamentals course; however, the actual practice is offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

BSC Decontamination is referenced but not explored, in depth, in the BSC Fundamentals course. Due to its complexities, this is considered a more advanced skill. The BSC Decontamination instruction and practical are offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

If you have questions, please contact the EAB Training Center Manager, Derek Glenn at dglenn@eabcoinc.com.

Who should Attend:
  • Field certification professionals
  • Laboratory safety and compliance personnel
  • HVAC and TAB professionals working in controlled environments
  • Industrial hygiene professionals
  • Architects and Design Engineers
  • Professionals that utilize BSCs
  • Equipment Manufacturers
  • Facility Engineers and Managers
  • Those pursuing NSF/ANSI 49 certification
  • BioSafety and EHS Professional
Prerequisites: None
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 15 Hours
eLearning: Online LMS Resources for six months
CE Credits: 4 credits

Upcoming Dates

  • May 18, 2026 - May 21, 2026 

  • September 28, 2026 - October 1, 2026 

The class schedule is as follows.

Day 1: 7:30 AM – 5:00 PM
Day 2: 7:30 AM – 5:00 PM
Day 3: 7:30 AM – 5:00 PM
Day 4: 7:30 AM – 4:30 PM

Breakfast and Lunch provided.

Learn More

To learn more about our training programs or to register to attend, please contact our Training Center Manager, Derek Glenn.

Contact Our Training Center Manager

Biological Safety Cabinet Certification Fundamentals

The Biological Safety Cabinet (BSC) Certification Fundamentals course is designed to equip professionals with the essential knowledge and hands-on experience required to perform field certification tests on biosafety cabinets. This course establishes the foundation for all professionals in the industry, ensuring a standardized approach to best practices. Additionally, it offers owners valuable insights into the proper certification process, helping make informed decisions about quality and compliance. This course provides participants with eight (8) completed test reports, including tests from B2 cabinets, ensuring they meet partial prerequisites for advanced certification.

Throughout the course, participants will gain expertise in:

  • Fundamentals of BSC components and operation
  • Discuss the purpose and function of various types of BSCs and HEPA filters
  • Proper BSC Install, Setup, Cleaning and Disinfecting
  • Documenting certification reports in compliance with NSF/ANSI 49 standards
  • Performing measurements and calculations
  • Understanding airflow dynamics and critical performance criteria for BSC certification
  • Applying safety and regulatory best practices for laboratory environments
  • BSC Design and Construction
  • BSC Lifespan and Decommissioning process
  • Instructional and practical training on the following NSF Primary Tests:
    • o Downflow Velocity Test
    • o Inflow Velocity Test – DIM Method
    • o Inflow Velocity Test – Constricted Access Opening Method
    • o HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • o Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • o Site Installation Assessment Test
    • o Airflow Pattern Test
  • Overview discussion on the following NSF Secondary Tests:
    • o Vibration Test
    • o Lighting Intensity Test
    • o Noise Level Test

Calculating Inflow/Downflow Velocity From Volume – TypeB2 Cabinet is introduced in the BSC Fundamentals course; however, the actual practice is offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

BSC Decontamination is referenced but not explored, in depth, in the BSC Fundamentals course. Due to its complexities, this is considered a more advanced skill. The BSC Decontamination instruction and practical are offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

If you have questions, please contact the EAB Training Center Manager, Derek Glenn at dglenn@eabcoinc.com.

Who Should Attend:

  • Field certification professionals
  • Laboratory safety and compliance personnel
  • HVAC and TAB professionals working in controlled environments
  • Industrial hygiene professionals
  • Architects and Design Engineers
  • Professionals that utilize BSCs
  • Equipment Manufacturers
  • Facility Engineers and Managers
  • Those pursuing NSF/ANSI 49 certification
  • BioSafety and EHS Professional
Prerequisites: None
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 15 Hours
eLearning: Online LMS Resources for six months
CE Credits: 4 credits
Throughout the course, participants will gain expertise in:
  • Fundamentals of BSC components and operation
  • Discuss the purpose and function of various types of BSCs and HEPA filters
  • Proper BSC Install, Setup, Cleaning and Disinfecting
  • Documenting certification reports in compliance with NSF/ANSI 49 standards
  • Performing measurements and calculations
  • Understanding airflow dynamics and critical performance criteria for BSC certification
  • Applying safety and regulatory best practices for laboratory environments
  • BSC Design and Construction
  • BSC Lifespan and Decommissioning process
  • Instructional and practical training on the following NSF Primary Tests:
    • Downflow Velocity Test
    • Inflow Velocity Test – DIM Method
    • Inflow Velocity Test – Constricted Access Opening Method
    • HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • Site Installation Assessment Test
    • Airflow Pattern Test
  • Overview discussion on the following NSF Secondary Tests:
    • Vibration Test
    • Lighting Intensity Test
    • Noise Level Test

Calculating Inflow/Downflow Velocity From Volume – TypeB2 Cabinet is introduced in the BSC Fundamentals course; however, the actual practice is offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

BSC Decontamination is referenced but not explored, in depth, in the BSC Fundamentals course. Due to its complexities, this is considered a more advanced skill. The BSC Decontamination instruction and practical are offered in the EAB Training NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course.

If you have questions, please contact the EAB Training Center Manager, Derek Glenn at dglenn@eabcoinc.com.

Who should Attend:
  • Field certification professionals
  • Laboratory safety and compliance personnel
  • HVAC and TAB professionals working in controlled environments
  • Industrial hygiene professionals
  • Architects and Design Engineers
  • Professionals that utilize BSCs
  • Equipment Manufacturers
  • Facility Engineers and Managers
  • Those pursuing NSF/ANSI 49 certification
  • BioSafety and EHS Professional
Prerequisites: None
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 15 Hours
eLearning: Online LMS Resources for six months
CE Credits: 4 credits

Upcoming Dates

  • May 18, 2026 - May 21, 2026 

  • September 28, 2026 - October 1, 2026 

The class schedule is as follows.

Day 1: 7:30 AM – 5:00 PM
Day 2: 7:30 AM – 5:00 PM
Day 3: 7:30 AM – 5:00 PM
Day 4: 7:30 AM – 4:30 PM

Breakfast and Lunch provided.

Learn More

To learn more about our training programs or to register to attend, please contact our Training Center Manager, Derek Glenn.

Contact Our Training Center Manager

Daily Agenda

Daily Agenda

Primary Reports that make a BSC Certification:

Primary Reports that make a BSC Certification:

Secondary Reports that can be included in a BSC Certification:

Secondary Reports that can be included in a BSC Certification:

Day One

8:30 am

Breakfast and Welcome

Standard Air and Density Correction

Ductwork Traversing and Capture Hoods

12:00 pm

Lunch

Correction Factors and Proportional Balancing

4:30 pm

Homework

Day Two

7:30 am

Breakfast and Homework Review

Flow Hood Measurement Hands On

Electrical Safety and NFPA 70E Hands On

11:30 am

Lunch

Basics of Electricity

Electrical Motors and VFDs

Day Three

7:30 am

Breakfast and Welcome

Rotational Speed Hands On

12:00 pm

Lunch

Sheave and Belt Sizing Hands On

Ductworks Systems and Devices

Fan Works

5:00 pm

Homework

Day Four

7:30 am

Breakfast and Welcome

Rotational Speed Hands On

12:00 pm

Lunch

AHU & RTU Data and Submittals Hands On

Water Systems

Pump Laws

Air Terminal Units

4:30 pm

Homework

Day Five

7:30 am

Breakfast and Welcome

Hydronics Hands On

12:00 pm

Lunch & Dismissal

Day One

7:30 am

Breakfast
Welcome Slide Deck
Introduction to BSCs, NSF 49, BSLs, BSC Classifications and Airflow Patterns
BSC Design, Construction, HEPA Filters, Memorandums

12:00 pm

Lunch

1:00 pm

BSC Design Variations
BSC Best Use, Practices, and Procedures
Proper BSC Install and Setup
Startup Procedure for a New BSC
Cleaning and disinfection of the BSC total work area
BSC Relocation
Day 1 Wrap-up and Q&A Session

5:00 pm

Day 1 Dismissal

Day Two

7:30 am

Breakfast / Review Day 1 / Overview of Day 2 Objectives
Instruments and Apparatuses
Hands-On Activity: Discover Instruments and Apparatuses
BSC Airflow Smoke Patterns
Hands-On Activity: Measure BSC Dimensions & Airflow Smoke Pattern Tests Practice

12:00 pm

Lunch

1:00 pm

Airflow Measurements – Downflow Velocity Test & Inflow Velocity Test DIM Method
Hands-On Activities: Downflow Velocity Test & Inflow Velocity Test DIM Method Practice
Airflow Measurements – Airflow Calculations
Day 2 Wrap-up and Q&A Session

5:00 pm

Day 2 Dismissal

Day Three

7:30 am

Breakfast / Review Day 2 / Overview of Day 3 Objectives
BSC Exhaust Flow Measurements & Concurrent Balance Values (CBV)
Measure Filter Load
Hands-On Activities: Exhaust Flow Measurements, CBV, Filter Load Practice
BSC Airflow Monitor, Blower Interlocks, and Site Installation Assessment Test
Hands-On Activities: Site Installation Assessment Test Practice

12:00 pm

Lunch

1:00 pm

HEPA Filter Leak Test – Scan Method – Type A & Probe Method – Type B
Hands-On: HEPA Filter Leak Test – Scan Method – Type A & Probe Method – Type B Practice
BSC Certification Reporting
Secondary Testing Documentation
BSC Lifespan Decommissioning
Day 3 Wrap-up and Q&A Session

5:00 pm

Day 3 Dismissal

Day Four

7:30 am

Breakfast / Review Day 1 / Overview of Day 4 Objectives
Hands-On Activities: BSC Certification and Reporting
QA Review for BSC Reports

12:00 pm

Lunch

1:00 pm

Hands-On Activities: BSC Certification and Reporting (continued)
Q&A Review for BSC Reports
Day 4 Wrap-up and Q&A Session – Course Complete

4:30 pm

Dismissal

Training Course - Biological Safety Cabinet Certification Fundamentals

At EAB, our team of experts are uniquely equipped to train the next generation of Critical Environment Certification Professionals

Emilio Rosales

CEC Service Manager

Getting Here

For further travel details, please visit our travel page

Address

604 Spring Hill Dr Ste 140, Spring, TX 77386

Phone

Email

Contact Us