NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training Course

NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training Course

Welcome to
EAB Training!

We’re thrilled to embark on this journey together! This page serves as your comprehensive guide to all the essential information you need before attending EAB Training.

As always, if you have any other questions, please reach out to our
Training Center Manager, Derek Glenn, at dglenn@eabcoinc.com.

What's Included in the Course?

– 5 Days of Instruction and 2 Days of Testing

– Resource Materials

– Instrumentation Instruction

– Breakfast and Lunch on Instructional Days

What's Included in the Course?

– 5 Days of Instruction and 2 Days of Testing

– Resource Materials

– Instrumentation Instruction

– Daily Breakfast and Lunch

What Should I Bring
to EAB Training?

• Computer or tablet with internet capabilities to access the EAB Learning Management System (LMS).

• A calculator that is not on a computer, tablet, or smart device.

• Your personal copy of the NSF/ANSI 49 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification Standard if you have one.

Prerequisites: Click here for more info

What Should I Bring
to EAB Training?

• Computer or tablet with internet capabilities to access the EAB Learning Management System (LMS).

• A calculator that is not on a computer, tablet, or smart device.

• Your personal copy of the NSF/ANSI 49 Biosafety Cabinetry: Design, Construction, Performance, and Field Certification Standard if you have one.

Prerequisites: Click here for more info

What About Meals
and Snacks?

Breakfast, lunch, and snacks are included Monday – Friday for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

What About Meals
and Snacks?

Breakfast, lunch, and snacks are included Monday – Friday for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

What Should I Bring
to EAB Training?

What Should I Bring
to EAB Training?

A calculator, safety glasses, and safety gloves is all you will need for our classes. Once you are at EAB Training, everything else is provided…

A calculator, safety glasses, and safety gloves is all you will need for our classes. Once you are at EAB Training, everything else is provided…

What About Meals
and Snacks?

Breakfast, lunch, and snacks will be included for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

What About Meals
and Snacks?

Breakfast, lunch, and snacks will be included for EAB Training Students.

Please let us know if you have any dietary restrictions.

Email Derek Glenn at dglenn@eabcoinc.com.

NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training

NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training

The NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course offered by EAB Training is designed to instruct participants according to industry standards, providing comprehensive knowledge and hands-on experience in the field of Biosafety Cabinet (BSC) certification. Participants will gain in-depth understanding of BSC construction and functions, review various BSC types and their installation requirements, and learn best practices for using BSCs safely and effectively. This course prepares participants for the NSF 49 written and practical exams.

Participants will engage in practical exercises, including conducting site assessments and creating testing grids for certification. They will learn to perform critical tests such as inflow and downflow airflow tests, smoke pattern tests, and balancing airflow tests.

The curriculum includes:

  • Overview of Biological Safety Cabinets
  • Introduction to Data Sheets and Certification
  • Instructional and practical training on the following:
    • o Downflow Velocity Test
    • o Inflow Velocity Test – DIM Method
    • o Inflow Velocity Test – Constricted Access Opening Method
    • o Calculating Inflow/Downflow Velocity from Volume – Type B2 Cabinet
    • o HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • o Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • o Vibration Test
    • o Lighting Intensity Test
    • o Noise Level Test
    • o Site Installation Assessment Test
    • o Airflow Pattern Test
    • o Cabinet Gas Decontamination (Mock)

There is a separate application process and fee for the NSF exam, and EAB Training does not guarantee your participation in the NSF exam. NSF exam arrangements are required at least 30 days in advance with NSF directly.

Prerequisites: Click here for more info
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 20 Hours
eLearning: Online LMS Resources for six months
NSF CE Credits: 5 Credits
Examination Time: Determined by the NSF proctor
The curriculum includes: This course covers the following topics:
  • Overview of Biological Safety Cabinets
  • Introduction to Data Sheets and Certification
  • Instructional and practical training on the following:
    • Downflow Velocity Test
    • Inflow Velocity Test – DIM Method
    • Inflow Velocity Test – Constricted Access Opening Method
    • Calculating Inflow/Downflow Velocity from Volume – Type B2 Cabinet
    • HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • Vibration Test
    • Lighting Intensity Test
    • Noise Level Test
    • Site Installation Assessment Test
    • Airflow Pattern Test
    • Cabinet Gas Decontamination (Mock)
Prerequisites: Click here for more info
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 20 Hours
eLearning: Online LMS Resources for six months
NSF CE Credits: 5 Credits
Examination Time: Determined by the NSF proctor

Upcoming Dates

  • April 6, 2026 - April 12, 2026 

  • October 19, 2026 - October 25, 2026 

  • November 3, 2025 - November 9, 2025 

The class schedule is as follows.

Monday – Friday: Instructional Training / Saturday - Sunday: Written exam and performance certification in the presence of a testing proctor

Breakfast and Lunch provided.

Learn More

To learn more about our training programs or to register to attend, please contact our Training Center Manager, Derek Glenn.

Contact Our Training Center Manager

There is a separate application process and fee for the NSF exam, and EAB Training does not guarantee your participation in the NSF exam. NSF exam arrangements are required at least 30 days in advance with NSF directly.

NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training

The NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training course offered by EAB Training is designed to instruct participants according to industry standards, providing comprehensive knowledge and hands-on experience in the field of Biosafety Cabinet (BSC) certification. Participants will gain in-depth understanding of BSC construction and functions, review various BSC types and their installation requirements, and learn best practices for using BSCs safely and effectively. This course prepares participants for the NSF 49 written and practical exams.

Participants will engage in practical exercises, including conducting site assessments and creating testing grids for certification. They will learn to perform critical tests such as inflow and downflow airflow tests, smoke pattern tests, and balancing airflow tests.

The curriculum includes:

  • Overview of Biological Safety Cabinets
  • Introduction to Data Sheets and Certification
  • Instructional and practical training on the following:
    • o Downflow Velocity Test
    • o Inflow Velocity Test – DIM Method
    • o Inflow Velocity Test – Constricted Access Opening Method
    • o Calculating Inflow/Downflow Velocity from Volume – Type B2 Cabinet
    • o HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • o Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • o Vibration Test
    • o Lighting Intensity Test
    • o Noise Level Test
    • o Site Installation Assessment Test
    • o Airflow Pattern Test
    • o Cabinet Gas Decontamination (Mock)

There is a separate application process and fee for the NSF exam, and EAB Training does not guarantee your participation in the NSF exam. NSF exam arrangements are required at least 30 days in advance with NSF directly.

Prerequisites: Click here for more info
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 20 Hours
eLearning: Online LMS Resources for six months
NSF CE Credits: 5 Credits
Examination Time: Determined by the NSF proctor
The curriculum includes: This course covers the following topics:
  • Overview of Biological Safety Cabinets
  • Introduction to Data Sheets and Certification
  • Instructional and practical training on the following:
    • Downflow Velocity Test
    • Inflow Velocity Test – DIM Method
    • Inflow Velocity Test – Constricted Access Opening Method
    • Calculating Inflow/Downflow Velocity from Volume – Type B2 Cabinet
    • HEPA Filter Leak Test – Type A Cabinet (Scan Method)
    • Exhaust HEPA Filter Leak Test – Type B Cabinet (Probe Method)
    • Vibration Test
    • Lighting Intensity Test
    • Noise Level Test
    • Site Installation Assessment Test
    • Airflow Pattern Test
    • Cabinet Gas Decontamination (Mock)
Prerequisites: Click here for more info
Classroom Instruction Time: 15 Hours
Hands-On Instruction Time: 20 Hours
eLearning: Online LMS Resources for six months
NSF CE Credits: 5 Credits
Examination Time: Determined by the NSF proctor

Upcoming Dates

  • April 6, 2026 - April 12, 2026 

  • October 19, 2026 - October 25, 2026 

  • November 3, 2025 - November 9, 2025 

The class schedule is as follows.

Monday – Friday: Instructional Training / Saturday - Sunday: Written exam and performance certification in the presence of a testing proctor

Breakfast and Lunch provided.

Learn More

To learn more about our training programs or to register to attend, please contact our Training Center Manager, Derek Glenn.

Contact Our Training Center Manager

There is a separate application process and fee for the NSF exam, and EAB Training does not guarantee your participation in the NSF exam. NSF exam arrangements are required at least 30 days in advance with NSF directly.

Daily Agenda

Daily Agenda

Agenda based on the NSF 49 Workflow Order of Operations

Agenda based on the NSF 49 Workflow Order of Operations

Day One

8:30 am

Breakfast and Welcome

Standard Air and Density Correction

Ductwork Traversing and Capture Hoods

12:00 pm

Lunch

Correction Factors and Proportional Balancing

4:30 pm

Homework

Day Two

7:30 am

Breakfast and Homework Review

Flow Hood Measurement Hands On

Electrical Safety and NFPA 70E Hands On

11:30 am

Lunch

Basics of Electricity

Electrical Motors and VFDs

Day Three

7:30 am

Breakfast and Welcome

Rotational Speed Hands On

12:00 pm

Lunch

Sheave and Belt Sizing Hands On

Ductworks Systems and Devices

Fan Works

5:00 pm

Homework

Day Four

7:30 am

Breakfast and Welcome

Rotational Speed Hands On

12:00 pm

Lunch

AHU & RTU Data and Submittals Hands On

Water Systems

Pump Laws

Air Terminal Units

4:30 pm

Homework

Day Five

7:30 am

Breakfast and Welcome

Hydronics Hands On

12:00 pm

Lunch & Dismissal

Day One

7:30 am

Breakfast
Welcome Slide Deck and Introductions
NSF 49 Introduction
BSC Cabinet Classification Types and Airflow Patterns
Biological Safety Levels (BSL) Overview
HEPA filter types
Design, Construction, and Memorandums
Sample NSF Data Sheet Overview
Common Failure Scenario Introduction
Instrumentation and Equipment Overview
Apparatus Testing Equipment and Calibration Overview

12:00 pm

Lunch

1:00 pm

Downflow Velocity Test – Discussion and Data Sheet Detail
Laboratory Area Tour
Downflow Velocity Test – Lab Practice Practical
Inflow Velocity Test (DIM Method) – Discussion and Data Sheet Detail
Inflow Velocity Test (DIM Method) – Lab Practice Practical
Inflow Velocity Test (Constricted Method) – Discussion and Data Sheet Detail
Inflow Velocity Test (Constricted Method) – Lab Practice Practical
Day 1 Wrap-up and Q&A Session

5:00 pm

Day 1 Dismissal

Day Two

7:30 am

Breakfast / Review Day 1 / Overview of Day 2 Objectives
Calculating Downflow / Inflow Velocity From Volume (Type B2)-Discussion and Data Sheet Detail
Calculating Downflow / Inflow Velocity From Volume (Type B2) – Lab Practice Practical
HEPA Filter Leak Test – Type A (Scan Method) – Discussion and Data Sheet Detail
HEPA Filter Leak Test – Type A (Scan Method)

12:00 pm

Lunch

1:00 pm

Exhaust HEPA Filter Leak Test-Type B (Probe Method)-Discussion and Data Sheet Detail
Exhaust HEPA Filter Leak Test – Type B (Probe Method)
Site Installation Assessment Test – Discussion and Data Sheet Detail
Site Installation Assessment Test – Lab Practice Practical
Airflow Smoke Pattern Testing – Discussion and Data Sheet Detail
Airflow Smoke Pattern Testing – Lab Practice Practical
Day 2 Wrap-up and Q&A Session

5:00 pm

Day 2 Dismissal

Day Three

7:30 am

Breakfast / Review Day 2 / Overview of Day 3 Objectives
Light Intensity Test – Discussion and Data Sheet Detail
Light Intensity Test – Lab Practice Practical
Vibration Testing – Discussion and Data Sheet Detail
Vibration Testing – Lab Practice Practical
Noise Level Testing – Discussion and Data Sheet Detail
Noise Level Testing – Lab Practice Practical

12:00 pm

Lunch

1:00 pm

Decontamination Procedures – Discussion and Data Sheet Detail
Decontamination Procedures – Mock Lab Practice Practical

5:00 pm

Day 3 Wrap-up and Q&A Session

Day Four

7:30 am

Breakfast / Review Day 3 / Overview of Day 4 Objectives

Mock Practical Exams (Lunch will be provided)

1. Downflow Velocity

2. Inflow Velocity Test – DIM Method

3. Inflow Velocity Test – Constricted Method

4. Calculating Downflow / Inflow velocity From Volume (Type B2)

5. HEPA Filter Leak Test – Scan Method (Type A)

6. Exhaust HEPA Filter Leak Test – Probe Method

7. Site Installation Assessment Test

8. Airflow Smoke Patterns Test

9. Lighting Intensity Test

10. Vibration Test

11. Noise Level Test

Day 4 Wrap-up and Q&A Session

5:00 pm

Day 4 Dismissal

Day Five

7:30 am

Breakfast / Review Day 4 / Overview of Day 5 Objectives

Mock Practical Exam – Continue

12. Decontamination Test

12:00 pm

Lunch & Study

12:30 pm

Prepare Testing Room (If applicable)

1:00 pm

NSF Proctored Written Exam (If applicable)
Review for Next Day NSF Proctored Practical Exam & Exit Surveys

Day Six

7:30 am

EAB Testing Site – Students & Proctors are responsible for their own food and necessities

Group 1 NSF Proctored Practical Exam (If applicable)

Day 6 Wrap-up and Clean-up

6:30 pm

Day 6 Practical Testing Day Dismissal

Day Seven

7:30 am

EAB Testing Site – Students & Proctors are responsible for their own food and necessities

Group 1 NSF Proctored Practical Exam (If applicable)

Day 7 Wrap-up and Clean-up

6:30 pm

Day 7 Practical Testing Day Dismissal

Training Course - NSF/ANSI 49 Biological Safety Cabinet Field Certifier Advanced Training

At EAB, our team of experts are uniquely equipped to train the next generation of Critical Environment Certification Professionals.

Emilio Rosales

CEC Service Manager

Getting Here

For further travel details, please visit our travel page

Address

604 Spring Hill Dr Ste 140, Spring, TX 77386

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